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KMID : 0861120040080020081
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Korean Journal of Oriental Preventive Medicine
2004 Volume.8 No. 2 p.81 ~ p.98
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Effects of Saururus Chinensis Baill Extracts on Anticancer Activity and Cadmium Induced Cytotoxicity
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Park Sung-Jung
Yu Hyun-Ju
Choi Hee-Seok
Seo Byeong-Yun
Yang Seon-Ho
Kim Yeong-Hun
Jeong Jae-Yeal
Lee Ki-Nam
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Abstract
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1. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus chinensis Baill. showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Baill. possessed a potential antitumorous agent. 2. In vitro the antitoxic activity of ethanol extract of Saururus Chinensis Bail on NIH 3T3 fibroblasts was evaluate by the MTT (3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl-2H-tetra캐lium bromide) and SRB (sulforhodamine B protein) assays. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that Saururus Chinensis Bail extract retains a potential antitoxic activity. 3. Complexation of Cd (II) ion with ligands such as quercetin has been determined by UV-vis spectrophotometric method in tris buffer solution at various pH. It was found that only 1 : 1 Cd-complex is formed by the interaction between catechol moiety in ring B and cadmium [Cd(II)] in aqueous solution. The spectral parameters for Cd-complex were determined by Beer’s law at various pH. It has shown that 1 : 1 Cd-complex has a maximum absorbance and red shift by the alkaline pH.
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KEYWORD
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Saururus Chinensis Baill Extracts, Anticancer Activity, Cadmium Induced Cytotoxicity
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